Corticotropin releasing hormone and related peptides can act as bioregulatory factors in human keratinocytes |
| |
| Authors: | Andrzej T Slominski Birgit Roloff Blazej Zbytek Edward T Wei Klaus Fechner Jonathan Curry Jacobo Wortsman |
| |
| Institution: | (1) Department of Pathology, Medical Center, Loyola University, 2160 First South Avenue, 60153 Maywood, Illinois;(2) Department of Histology and Immunology, Medical University of Gdansk, Gdansk, Poland;(3) Department of Pharmacology, School of Public Health, University of California, Berkeley, California;(4) Institute of Molecular Pharmacology, Berlin, Germany;(5) Department of Medicine, Southern Illinois University, Springfield, Illinois |
| |
| Abstract: | Summary Following previous findings in human skin of the functional expression of genes for the corticotropin releasing hormone (CHR)
receptor type 1 (CRH-R1) and CRH itself, we searched for local phenotypic effects for peptides related to CRH. We now report
that CRH, sauvagine, and urocortin inhibit proliferation of human HaCaT keratinocytes in a dose-dependent manner. The peptides
produced variable cyclic adenosine 3′∶5′-monophosphate stimulation with CRH having the highest potency. Binding of iodine
125 CRH to intact keratinocytes was inhibited by increasing doses of CRH, sauvagine, or urocortin, all showing equal inhibitory
potency. Immunocytochemistry identified CRH-R1 immunoreactivity in HaCaT keratinocytes. In conclusion, CRH (exogenous or produced
locally) and the related urocortin and sauvagine peptides can modify human keratinocyte phenotype through a receptor-mediated
pathway. |
| |
| Keywords: | CRH urocortin sauvagine CRH receptors keratinocytes skin |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
