Callus growth and somatic embryogenesis in thalamus tissue ofRanunculus asiaticus L. CultivatedIn vitro: Cytokinin effect and phenol metabolism |
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| Authors: | Margherita Beruto Paolo Curir Pierre Debergh |
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| Institution: | (1) Istituto Regionale per la Floricoltura, Via Carducci, 12, 18038 Sanremo (IM), Italy;(2) University, Ist. Chimica Agraria, V. P. Giuria, 15, 10100 Torino, Italy;(3) Department of Plant Production, Horticulture, University of Gent, Coupure links, 653, B9000 Gent, Belgium |
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| Abstract: | Summary The effect of cytokinin on growth and plant regeneration of thalamus-derived calluses ofRanunculus asiaticus L. has been investigated with various concentrations of 6-benzyladenine and 6-furfurylaminopurine (kinetin), in a medium
containing 2,4-dichlorophenoxyacetic acid levels, which was decreased to 0 over three subcultures. Cytokinins, although not
essential, for initiating callus production, improved subsequent callus growth and plant regeneration. No somatic embryogenesis
was observed on calluses grown on media lacking cytokinins or containing only kinetin. Calluses manifested embryogenesis on
media containing 6-benzyladenie plus kinetin or only 6-benzyladenine. Nondifferentiating callus was characterized by a high
content of phenolic polymers and an elevated peroxidase and polyphenol oxidase activity in comparison with differentiating
callus. Differences in simple phenol concentrations were observed in the two kinds of callus. |
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| Keywords: | 6-benzyladenine phenolic polymers peroxidase polyphenol oxidase tissue culture |
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