设计性细胞生物学实验探索--肿瘤细胞的耐药性分析

肿瘤多药耐药是临床上肿瘤化疗失败的主要原因,国内外对多药耐药机制的研究已有多年历史。肿瘤细胞的耐药性状分析是研究耐药机制的基础工作,其实验思路和方法多样且较为成熟。该实验教学改革项目以"肿瘤细胞的耐药性分析"为主旨设计细胞生物学实验项目,通过全开放式的实验教学模式激发学生从细胞形态学、药物敏感性、耐药相关蛋白表达及功能等各方面,对野生型人乳腺癌MCF-7/WT细胞和耐阿霉素人乳腺癌MCF-7/ADM细胞的耐药性进行分析。学生以小组为单位进行自主调研并设计实验方案,在开放的本科实验教学平台开展实验,最后通过多样化的考核模式进行实验总结与评价。该文通过具体实施例进行实验结果展示,并从整体的方案设计、结果统计和课后满意度调查三方面进行实践总结和反思。该实验教学项目整体内容成熟、安全、稳定,具有一定的创新性、实用性和可操作性,对于培养学生的主动性和创造性,提高学生的综合实验能力具有重要的作用。

Exploration of the Designed Experiment of Cell Biology--Drug Resistance Analysis of Tumor Cells

Multi-drug resistance is the main reason for the failure of tumor chemotherapy in clinic,and its mechanism has been studied for many years.Drug resistance analysis of tumor cells is the basic work to study the mechanism of drug resistance.Its experimental ideas and methods are diverse and mature.During the open experimental teaching mode,students are encouraged to analyze the drug resistance of wild-type human breast cancer MCF-7/WT cells and adriamycin-resistant human breast cancer MCF-7/ADM cells from the aspects of cell morphology,drug sensitivity and expression of drug-resistant related proteins.First,students conduct independent research and design experimental programs in groups,then practice in the open undergraduate experimental teaching platform,finally summarize and evaluate experiments through diversified assessment modes.This paper presents the results through a specific embodiment,and makes a practical summary and reflection from the three aspects of overall scheme design,result statistics and after-class satisfaction survey.The whole experimental system is mature,safe and stable,with certain innovation,practicability and operability,which plays an important role in cultivating students’initiative and creativity,improving their comprehensive experimental ability.