精原干细胞技术研究进展

精原干细胞(spermatogonial stem cells,SSCs)是位于睾丸曲细精管基膜上能自我更新和连续分化产生精子的最原始精原细胞,是雄性体内唯一能将遗传信息自然传至子代并可终生复制的双倍体细胞,对复杂的精子发生过程有着至关重要的作用。作为一个未分化细胞群体,SSCs在精子生成和物种进化所必需的基因传递中发挥作用。基于课题组多年的研究,该文较系统地评述了SSCs的生物学特性、分离富集、体外培养影响因素和移植技术等方面的进展,以期对雄性辅助生殖、细胞再生治疗、畜牧业生产等研究应用提供借鉴。

Advances in the Research of Spermatogonial Stem Cell Techniques

SSCs(spermatogonial stem cells)are the most primitive spermatogonial cells located on the basement membrane of the seminiferous tubules in the testis,which can self-renew and differentiate continuously to produce sperm.SSCs are the only replicable diploid cells which can transfer genetic information into offspring naturally in male body throughout life.They play an important role in the complex spermatogenesis process.As an undifferentiated cell population,SSCs have the role of gene transfer necessary for the generation of male gametes and species evolution.Based on our previous research,the biological characteristics,isolation and enrichment,factors affecting in vitro culture and transplantation technology of SSCs are systematically reviewed,which has theoretical significance for the research and application of male assisted reproduction,cell regeneration therapy as well as animal husbandry production.