环状RNA circCapzb在早孕小鼠围植入期子宫内膜中的表达

环状RNA(circular RNA,circRNA)是一类新型内源性非编码RNA,与多种疾病的发生、发展密切相关,但在胚胎着床的过程中罕见报道。该文旨在探讨环状RNA circCapzb在早孕小鼠围植入期子宫内膜中的表达。采用Real-time PCR检测正常妊娠小鼠孕第5天(d5)至第7天(d7)胚胎着床点及胚胎着床旁组织中circCapzb的表达水平;分别构建小鼠体内人工诱导蜕膜化模型和原代小鼠子宫内膜基质细胞体外人工诱导蜕膜化模型,采用Real-time PCR分别检测circCapzb在组织及细胞蜕膜化诱导模型中的表达;通过生物信息学预测circCapzb下游靶miRNA:miR-377-3p和miR-7005-5p,并采用Real-time PCR检测其在蜕膜化诱导模型中的表达。结果表明,circCapzb在小鼠孕第5天至第7天胚胎着床点的表达明显高于着床旁;circCapzb在组织及体内外细胞蜕膜化诱导模型中诱导组的表达明显高于未诱导组(对照组);circCapzb下游靶miR-377-3p和miR-7005-5p在组织及体内外细胞蜕膜化诱导模型中诱导组的表达明显低于未诱导组。该研究初步表明,circCapzb在小鼠早孕期胚胎着床点高表达,在组织及体内外细胞蜕膜化诱导模型中高表达,在小鼠妊娠早期子宫内膜蜕膜化过程中可能发挥作用,但具体机制有待进一步研究。

Expression of circCapzb in Endometrium during Peri-Implantation Period of Early Pregnancy Mice

CircRNA (circular RNA),as a new class of endogenous non-coding RNAs,though closely related to the occurrence and progression of various diseases,is rarely investigated during embryo implantation.The purpose of this study was to investigate the expression of circCapzb in peri-implantation period endometrium of pregnant mice.The expression levels of circCapzb in embryo tissues in IS (implantation sites) and IIS (inter implantation sites) were detected by Real-time PCR from day 5 (d5) to day 7 (d7) of pregnancy.In vivo model of induced decidualization in mice and in vitro model of induced decidualization in primary mice endometrial stromal cells were constructed respectively,and the expression of circCapzb in the models was detected by Real-time PCR.The circCapzb downstream targets miRNAs were miR-377-3p and miR-7005-5p.They were predicted by bioinformatics,and their expression in the decidual induction model was detected by Real-time PCR.The results showed that circCapzb expression was significantly higher at the implantation sites from day 5 to day 7 than at the interimplantation sites.CircCapzb expression in tissue and cell decidualization induction in vivo and in vitro model was significantly higher in the induced group than in the non-induced group.The expression of miR-377-3p and miR-7005-5p downstream targets of circCapzb in the tissue and cell decidualization induction in vivo and in vitro model was significantly lower in the induced group than in the non-induced group.This study showed that circCapzb was highly expressed in mice embryo implantation sites during early pregnancy,and in tissue and cell decidualization in vivo and in vitro induction models.circCapzb might play a role in mouse endometrial decidualizationduring early pregnancy,but the specific mechanism remained to be further studied.