| 德国小蠊生活史各期蛋白质、糖与酯酶同功酶的比较研究 |
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| 引用本文： | 邹文秀,高晓红,李玉兰,朱昌亮,叶炳辉.德国小蠊生活史各期蛋白质、糖与酯酶同功酶的比较研究[J].华东昆虫学报,1996,5(2):65-68. |
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| 作者姓名： | 邹文秀 高晓红 李玉兰 朱昌亮 叶炳辉 |
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| 作者单位： | [1]南京铁道医学院寄生虫学教研室，南京210009 [2]南京医科大学寄生虫学教研室，南京210029 |
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| 摘 要： | 德国小蠊生活史各期经等电聚焦后，用考马斯亮兰染蛋白质，然后用薄层层析扫描仪扫描，卵、若虫、成虫分别显带36、30和43条；用PAS染糖，分别显带29、21和33条。各阶段所显区带由糖蛋白、蛋白质和多糖组成。另补，本还对各阶段的酯酶同功酶进行了比较．用坚牢兰染色后．卵、若虫、成虫分别显带23、23和25条。结果表明．每种物质均以成虫期显带较多。
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| 关 键 词： | 酯酶同功酶 若虫 显带 成虫期 生活史 蛋白质 多糖 等电聚焦 糖蛋白 薄层层析 |
A COMPARATIVE RESEARCH ON THE PROTEIN,SUGAR AND ESTERASES ISOENZYME IN ALL STAGES OF BLATTELLA GERMANICA |
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| Zou Wenxiu.A COMPARATIVE RESEARCH ON THE PROTEIN,SUGAR AND ESTERASES ISOENZYME IN ALL STAGES OF BLATTELLA GERMANICA[J].Entomological Journal of East China,1996,5(2):65-68. |
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| Authors: | Zou Wenxiu |
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| Abstract: | By using isoelectric focusing(IEF) method,eggs,nymphs and adults of Blattella germanica showed 36,30 and 43 bands of proteins respectively through coomassie brilliant blue stain,and 29,21 and 33bands of sugar through PAS stain. All the above bands were proved to be composed of pure protein sugarprotein and polysaccharose,In addition,the esterase isoenzymes of all the above stages of Blattellagermanica were also compared in all stages and 23,23 and 25 bands were fouidrespectively. Adults showed more bands than other stages. |
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| Keywords: | Blattlella germanica IEF protein sugar esterases isoenzyme |
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