| 铁皮石斛内生真菌的分离及其挥发性成分分析 |
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| 引用本文： | 徐梦珂,印嘉闻,谈思源,倪寒,袁博,蒋继宏.铁皮石斛内生真菌的分离及其挥发性成分分析[J].氨基酸和生物资源,2017,39(1):53-57. |
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| 作者姓名： | 徐梦珂 印嘉闻 谈思源 倪寒 袁博 蒋继宏 |
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| 作者单位： | 江苏师范大学生命科学学院/江苏省药用植物生物技术重点实验室,江苏徐州,221116 |
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| 摘 要： | 铁皮石斛(Dendrobium officinale)是我国重要的濒危中药材,其药用价值和经济价值很高。以铁皮石斛为研究对象,采用组织块法,从铁皮石斛根、茎和叶中分离获得22株内生真菌。采用固相微萃取固定吸附(SPME),结合气相质谱(GCMS)解吸附分析技术对其中具有产香特性的3株内生真菌KLBMPD001、KLBMPD004和LBMPD009的挥发性成分进行分析。结果显示,这3株内生真菌均含有较高比例的棕榈酸,分别为37.94%、24.15%和48.55%,其中菌株KLBMPD001和KLBMPD009含有一定比例的硬脂酸,分别为12.64%和37.37%,只有菌株KLBMPD004含有8.74%的酞酸二丁酯。
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| 关 键 词： | 铁皮石斛 内生真菌 固相微萃取结合气相色谱-质谱分析技术(SPME-GC-MS) |
Isolation of Endophytic Fungi of Dendrobium officinale and Analysis of Their Volatile Constituents |
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| XU Mengke,YIN Jiawen,TAN Siyuan,NI Han,YUAN Bo,JIANG Jihong.Isolation of Endophytic Fungi of Dendrobium officinale and Analysis of Their Volatile Constituents[J].Amino Acids & Biotic Resources,2017,39(1):53-57. |
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| Authors: | XU Mengke YIN Jiawen TAN Siyuan NI Han YUAN Bo JIANG Jihong |
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| Institution: | ,The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province/School of Life Sciences,Jiangsu Normal University |
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| Abstract: | Dendrobium officinale is a famous endangered Chinese medicinal herb.It has extremely high pharmacological and economic values.22 endophytic fungi were isolated from the roots,stems and leaves of Dendrobium officinale by tissue block method.The volatile constituents of three fungi (KLBMPD001,KLBMPD004 and KLBMPD009) with aroma properties were analyzed.The results showed that all three endophytic fungi contained a high proportion of palmitic acid,the contents were 37.94％,24.15％ and 48.55％,respectively.Strain KLBMPD001 and KLBMPD009 contained a certain proportion of stearic acid,the contents were12.64 ％ and 37.37 ％ respectively.Only strain KLBMPD004 contained 8.74 ％ dibutyl phthalate. |
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| Keywords: | Dendrobium officinale endophytic fungi solid phase microextraction-gas chromatography-mass spectrometer (SPME-GC-MS) |
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