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Ribosomal RNA genes structure in somePopulus spp. (Salicaceae) and their hybrids
Authors:Renato D'Ovidio  Giuseppe Scarascia Mugnozza  Oronzo A. Tanzarella
Affiliation:(1) Dipartimento di Agrobiologia e Agrochimica, Universitá della Tuscia, via S. Camillo de Lellis, I-1100 Viterbo, Italy;(2) Dipartimento di Scienze dell' Ambiente Forestale e delle sue Risorse, Universitá della Tuscia, via S. Camillo de Lellis, I-1100 Viterbo, Italy
Abstract:The tandemly repeated multigene families encoding 18S and 25S rRNAs were studied at the restriction enzyme level inPopulus alba L.,Populus deltoidesBartr. exMarsh.,Populus trichocarpaTorr. & Gray and in the hybrids between the last two mentioned species. The analysis of single and double digestion with EcoRI, BamHI, XbaI, and SstI endonucleases showed the presence of single repetitive unit types of 12.25 and 11.75kb inP. alba andP. trichocarpa, respectively.P. deltoides showed two rDNA gene types having the same length (12.25Kb) but different nucleotide sequence in the IGS. The rDNAs genes ofP. deltoides andP. triochocarpa are inherited codominantly in their hybrids.
Keywords:Angiosperms  Salicaceae  Populus  Ribosomal RNA genes  restriction enzyme maps  rDNA
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