Effects of Density and Gating of Delayed-Rectifier Potassium Channels on Resting Membrane Potential and its Fluctuations |
| |
Authors: | S Marom H Salman V Lyakhov E Braun |
| |
Institution: | (1) The Rappaport Faculty of Medicine and the Faculty of Physics, Technion-Israel Institute of Technology, Haifa 32000, Israel, IL |
| |
Abstract: | The aim of this study is to evaluate directly, using a reduced experimental system, the nature of interactions between voltage-gated
potassium channels and the resting membrane potential. Xenopus oocytes were injected with various concentrations of cRNA coding for a delayed-rectifier potassium channel Shaker-IR. The effects of the density and kinetics of the expressed channels on resting membrane potential is explored in isolated
(``inside-out') patches. The channel density is given in terms of maximal conductance (G
max), measured from the maximal slope of the I-V curve under voltage clamp conditions. The capacitance of the experimental setup is approximately 1 pF. At high channel densities
(G
max > 10 pA/mV) the mean membrane potential is stabilized at approximately −60 mV. This resting membrane potential is more than
35 mV positive to the reversal potential for potassium ions under the same experimental conditions. Analyses of voltage clamp
experiments indicate that at high channel densities the mean membrane potential is determined by the rates of channel activation
and deactivation, but is not affected by the rates involved in the process of slow (C-type) inactivation. In contrast, at
lower channel densities membrane potential is very unstable, and its mean value and amplitude of fluctuations are strongly
affected by the process of slow (C-type) inactivation.
Received: 21 March 1996/Revised: 6 August 1996 |
| |
Keywords: | : Resting potential — Ion channel — Inactivation — Fluctuation — Xenopus oocyte |
本文献已被 SpringerLink 等数据库收录! |
|