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The subgenus Ashima (Diptera,Drosophildae, Phortica) from China,with DNA barcoding and descriptions of three new species
Authors:Keying An  Huiluo Cao  Xuelin Wang
Affiliation:1. Department of Entomology, South China Agricultural University, Tianhe, Guangzhou, 510642, China;2. Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China;3. Shenzhen Agricultural Science and Technology Promotion Center, Futian, Shenzhen, 518040, China
Abstract:In the present study, three new species found in Yunnan, Southwest China were described as members of the subgenus Phortica (Ashima): P. (A.) haba An & Chen, sp. nov., P. (A.) montipagana An & Chen, sp. nov. and P. (A.) qingsongi An & Chen, sp. nov. Barcode sequences (partial sequences of the mitochondrial COI gene) were collected from 61 specimens of 16 known and the above-mentioned three new Ashima species. The intra- and interspecific pairwise K-2P (Kimura two-parameter) COI distances were analysed and a phylogenetic tree was constructed based on the barcode sequences. Species delimitation in this subgenus was supported by integrating barcodes with morphological information, in particular for the three new species, considered to be cryptic species. In addition, the diversification of lineages in the subgenus Ashima was proposed to occur in southern China and adjacent areas, suggesting specific adaptation of Ashima species to the high plateau environments.

http://zoobank.org/urn:lsid:zoobank.org:pub:69A81D0E-5993-426D-9B59-A2AC5E52BD84
Keywords:biodiversity  cryptic species  drosophilid  morphology  taxonomy
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