Nanoproteomics comes of age |
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Authors: | Ying Zhu Paul D. Piehowski Ryan T. Kelly |
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Affiliation: | 1. Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratory , Richland, WA, USA https://orcid.org/0000-0002-5416-0566;2. Biological Sciences Division, Pacific Northwest National Laboratory , Richland, WA, USA;3. Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratory , Richland, WA, USA |
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Abstract: | ABSTRACT Introduction: Nanoproteomics, which is defined as quantitative proteome profiling of small populations of cells (<5000 cells), can reveal critical information related to rare cell populations, hard-to-obtain clinical specimens, and the cellular heterogeneity of pathological tissues. Areas covered: We present a brief review of the recent technological advances in nanoproteomics. These advances include new technologies or approaches covering major areas of proteomics workflow ranging from sample isolation, sample processing, high-resolution separations, to MS instrumentation. Expert commentary: We comment on the current state of nanoproteomics and discuss perspectives on both future technological directions and potential enabling applications. |
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Keywords: | Nanoproteomics single cell proteomics nanoPOTS |
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