Hybridoma antibody content and production rate in continuous culture: Effect of dilution rate |
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Authors: | Sridhar Reddy William M Miller |
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Institution: | (1) Department of Chemical Engineering, Northwestern University, 60208-3120 Evanston, Illinois |
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Abstract: | Summary Hybridoma IND1 viability was 95% for dilution rates (D) ranging from 45 to 100% of max (0.037 hr–1). Over this range, the cell concentration and total protein content increased with D. Washout occurred at D=0.041 hr–1, but the intracellular protein content continued to increase. The high- and low-content modes of the intracellular antibody distribution did not vary with D. The fraction of cells with high antibody content decreased with time, except for an increase at D=0.041 hr–1. This decrease did not affect the specific antibody production rate, which, like the high- and low-content modes, was independent of D. |
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