Determination of estriol, estriol sulfate, progesterone and neutral steroid mono- and disulfates in umbilical cord blood plasma |
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Authors: | T Laatikainen J Peltonen P Nylander |
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Institution: | Departments of Obstetrics and Gynecology, University Central Hospital, Helsinki, Finland |
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Abstract: | Fractions of unconjugated steroids, and steroid mono- and disulfates were isolated from cord plasma, and the concentrations of estriol, estriol sulfate, progesterone, 13 neutral steroid monosulfates (MoS) and 10 neutral steroid disulfates (DiS) were determined by gas-liquid chromatography. The mean concentrations in 30 cord plasma samples at term after normal pregnancy and delivery were as follows (μg/100 ml of free steroid ±standard deviation): estriol 16±5; estriol monosulfate 135±43; progesterone 59±19; dehydroepiandrosterone MoS 76±23; 5-androstene-3β,17α-diol DiS 279±77; 5-androstene-3β,17β-diol DiS 211±109; 16α-hydroxydehydroepiandrosterone MoS 305±97; 16β-hydroxy-dehydroepiandrosterone DiS 8±25; 33,17β-dihydroxy-5-androsten-16-one MoS 37±16, DiS 29±15 5-androstene-3β,16α,17β-triol MoS 25±9; 5-androstene-3β,16β,17α-triol DiS 31±14; pregnenolone MoS 4±33; 5-pregnene-3β,20α-diol MoS 41±14, DiS 68±43; 16α-hydroxypregnenolone MoS 101±42; 17-hydroxypregnenolone MoS 56±30; 21-hydroxypregnenolone DiS 26±15; 5-pregnene-3β,20α,21-triol MoS 37±18; 5α-pregnane-3α,20α-diol MoS 21±10, DiS 54±21; 5α-pregnane-3β,20α-diol MoS 18±9, DiS 7±39; 5β-pregnane-3α,20α-diol MoS 17±7; 5α-pregnane-3α,20α,21-triol MoS 110±56, DiS 22±19.The total amount of steroid monosulfates in the cord plasma pool was 1 mg/100 ml and that of steroid disulfates 0.5 mg/100 ml. 3β-Hydroxy-Δ5-steroids predominated. Considerable amounts of saturated c21 steroids were also detected. No statistically significant differences were found in the concentrations of any of the steroids studied, when a group of male and female fetuses were compared. |
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