| 外源钙对根际低氧胁迫下黄瓜幼苗ADH、LDH活性和同工酶的影响 |
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| 引用本文： | 王长义,郭世荣,杜长霞,刘超杰.外源钙对根际低氧胁迫下黄瓜幼苗ADH、LDH活性和同工酶的影响[J].生态学杂志,2010,29(4). |
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| 作者姓名： | 王长义 郭世荣 杜长霞 刘超杰 |
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| 作者单位： | 南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放试验室,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划项目(2009CB119000); 国家自然科学基金项目(30871736); 现代农业产业技术体系建设专项; 国家科技支撑计划项目(2008BADA6B07); 江苏省农业三项工程资助项目(SX(2008)026) |
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| 摘 要： | 以‘新泰密刺’黄瓜为材料,采用营养液栽培,外源使用Ca2+、钙离子通道抑制剂La3+与钙调素拮抗剂三氟拉嗪(TFP),研究了钙对根际低氧胁迫下黄瓜幼苗根系ADH、LDH活性和同工酶的影响。结果表明,低氧胁迫诱导产生了新的ADH和LDH同工酶条带。低氧胁迫下,ADH、LDH同工酶丰度和活性显著高于对照;外源增施Ca2+有利于Ca2+信号的形成和逆境信号的传递,营养液添加CaCl2缓解了低氧胁迫对黄瓜植株的伤害,ADH、LDH同工酶丰度和活性接近对照水平;La3+抑制Ca2+的吸收和体内运输,营养液添加LaCl3显著抑制了ADH和LDH同工酶丰度和酶活性,黄瓜幼苗植株生长受到抑制,生物量显著低于低氧处理,表明La3+加重了低氧胁迫对黄瓜幼苗植株的伤害;TFP抑制了低氧逆境胁迫信号的传递,营养液添加TFP抑制了ADH和LDH同工酶丰度和酶活性,ADH和LDH同工酶丰度和酶活性显著低于低氧处理,黄瓜幼苗植株生长受到抑制,黄瓜植株的低氧耐性降低。暗示外源Ca2+参与了低氧胁迫下黄瓜根系无氧呼吸代谢的调节,增强了Ca2+向植物体内的运输,缓解了低氧胁迫对黄瓜幼苗植株的伤害,增强了植物对低氧的耐性。
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| 关 键 词： | 低氧胁迫 钙 黄瓜幼苗 同工酶 |
Effects of exogenous calcium on the abundance and activity of ADH and LDH isoenzymes in cucumber seedlings root under root-zone hypoxia stress |
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| WANG Chang-yi,GUO Shi-rong,DU Chang-xia,LIU Chao-jie.Effects of exogenous calcium on the abundance and activity of ADH and LDH isoenzymes in cucumber seedlings root under root-zone hypoxia stress[J].Chinese Journal of Ecology,2010,29(4). |
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| Authors: | WANG Chang-yi GUO Shi-rong DU Chang-xia LIU Chao-jie |
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| Institution: | WANG Chang-yi,GUO Shi-rong,DU Chang-xia,LIU Chao-jie (College of Horticulture,Nanjing Agricultural University/Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China) |
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| Abstract: | A hydroponic experiment was conducted to study the effects of exogenous calcium,LaCl3,and trifluoperazine (TFP) on the abundance and activity of alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH) isoenzymes in cucumber seedlings under hypoxia stress.Hypoxia induced the formation of new ADH and LDH isoenzyme bands.Under hypoxia stress,the abundance and activity of ADH and LDH isoenzymes were significantly higher than those of the control.Increasing exogenous Ca2+ concentration was conducive to the C... |
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| Keywords: | hypoxia stress calcium cucumber seeding isoenzyme |
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