In vitro metabolic activation of ethidium bromide and other phenanthridinium compounds: mutagenic activity in Salmonella typhimurium. |
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| Authors: | J T Macgregor I J Johnson |
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| Affiliation: | Western Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Berkeley, California 94710, U.S.A. |
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| Abstract: | The induction of mutation by a variety of mutagens has been measured utilizing the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells (CHO/HGPRT) system). These mutagens include physical agents such as UV light and X-rays, and chemicals such as alkylating agents, ICR-191, and metallic compounds. This system can also be modified for study of the mutagenicity of promutagens such as dimethylnitrosamine (DMN) which require biotransformation for mutagenic action, either through the addition of a rat liver microsomal activation preparation or through a host-mediated activation step using Balb/c athymic mice. |
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| Keywords: | HX, hypoxanthine HGPRT, HX-guanine phosphoribosyl transferase F12FCM5, medium F12 containing 5% dialyzed fetal calf serum F12FCM10, medium F12 containing 10% dialyzed fetal calf 0erum TG, 6-thioguanine (2-amino, 6-mercaptopurine) TG mediu,. HX-free medium F12 containing 5% dialyzed fetal calf serum and 10 μM TG EMS, ethyl methanesulfonate DMN, dimethylnitrosamine |
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