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Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis * * |
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| Authors: | Qing Yang Xiaoming Xu Min Li Xiaodong Yuan Zhiguo Su Jan-Christer Janson Lijia An |
| Institution: | (1) Bioengineering Institute, Dalian University of Technology, Dalian, 116012, P.R. China;(2) Takara Biotech Dalian Co. Ltd., Dalian, 116600, P.R. China;(3) State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing, 100080, P.R. China;(4) Biosurface Science Center, Biomedical Center, 75 123 Uppsala, Sweden;(5) Bioengineering Institute, Dalian University of Technology, Dalian, 116012, P.R. China |
| Abstract: | The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l–1. |
| Keywords: | cDNA sequence defibrase expression separation thrombin-like enzyme |
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