| 体外诱导鸡胚胎生殖细胞分化为神经干细胞 |
| |
| 引用本文： | 王娟,潘效红,杜立新. 体外诱导鸡胚胎生殖细胞分化为神经干细胞[J]. 动物学报, 2008, 54(5) |
| |
| 作者姓名： | 王娟 潘效红 杜立新 |
| |
| 作者单位： | 1. 滨州医学院药学院生物技术教研室,烟台,264003
2. 中国农业科学院畜牧兽医研究所国家畜禽分子遗传育种中心,北京,100094 |
| |
| 摘 要： | 本研究探讨体外诱导鸡胚胎生殖细胞(EGCs)分化为神经干细胞(NSCs)的可能性.EGCs经类胚体(EB)阶段,以维生素A酸(RA)等进行诱导,在NSCs选择性培养基中筛培养扩增7 d,观察形态变化;采用RT-PCR法检测nestin基因表达及免疫细胞化学法检测nestin等NSCs特异性标志物,并对其扩增及分化能力进行观察.结果显示:EGCs经初级诱导,NSCs选择性培养基筛选培养7 d后,形成大量神经球样结构,可扩增传代;绝大部分神经球样结构呈nestin抗原阳性,表达nestin基因,且可分化为神经上皮样及少突胶质细胞.研究结果表明:RA等诱导的EGCs,经选择性培养基筛选培养可获得NSCs,有望为眼部神经变性疾病的治疗提供新的技术参考.
|
| 关 键 词： | 胚胎生殖细胞 神经干细胞 诱导 鸡 |
Differentiation of chicken embryonic germ cells into neural stem cells in vitro |
| |
| WANG Juan,PAN Xiao-Hong,DU Li-Xin. Differentiation of chicken embryonic germ cells into neural stem cells in vitro[J]. Acta Zoologica Sinica, 2008, 54(5) |
| |
| Authors: | WANG Juan PAN Xiao-Hong DU Li-Xin |
| |
| Affiliation: | WANG Juan1,PAN Xiao-Hong1,DU Li-Xin21.Department of Biotechnology,College of Pharmacy,Binzhou Medical University,Yantai 264003,Sh,ong,China2.National Center for Molecular , Breeding of Animal,Institute of Animal,Chinese Academy of Agricultural Sciences,Beijing 100094,China |
| |
| Abstract: | To explore the feasibility of inducing chicken embryonic germ cells into neural stem cells in vitro.Embryoid bodies(EB)induced by retinoic acid(RA),were selected in neural stem cell-defined medium for 7 days,and the resulting morphological changes were observed.The selected cells were stained immunocytochemically with anti-nestin antibodies,and their expansion and differentiation were analyzed.Large amounts of neurosphere-like colonies were derived from embryoid bodies in the selected medium on the 7th day,... |
| |
| Keywords: | Embryonic germ cells Neural stem cells Induct Chicken |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
