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Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel |
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| Authors: | Avi Eldar Sara Lawhon Paul F. Frelier Liliana Assenta Bruce R Simpson Patricia W Varner Herve Bercovier |
| Affiliation: | Department of Clinical Microbiology, The Hebrew University-Hadassah Medical School, POB 12272 Jerusalem 91010, Israel;Department of Poultry and Fish Diseases, The Kimron Veterinary Institute, Bet Dagan 50250, Israel;Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467, USA |
| Abstract: | Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178T (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates. |
| Keywords: | Restriction length polymorphism Streptococcus iniae Epidemiology 16S rDNA Ribotype |
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