Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification |
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Authors: | A Sakai S Kobayashi I Oiyama |
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Institution: | (1) Asabucho 1-5-23 Kitaku, 001 Sapporo, Japan;(2) Akitu Branch, Fruit Tree Research Station, Akitu, 729-24 Hiroshima, Japan |
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Abstract: | The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.Abbreviations DMSO
dimethyl sulfoxide
- PVS2
vitrification solution
- LN
liquid nitrogen
- DSC
differential scanning calorimeter
- BA
6-benzylaminopurine
- MT
Murashige-Tucker basal medium
- INAA
naphthaleneacetic acid |
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Keywords: | Cryopreservation Vitrification Nucellar cells Navel orange Citrus sinensis |
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