Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD+ regeneration |
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Authors: | Consuelo Vázquez-Limón Joel Vega-Badillo Alfredo Martínez Gabriela Espinosa-Molina Guillermo Gosset Xavier Soberón Agustín López-Munguía Joel Osuna |
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Affiliation: | (1) Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos, 62250, Mexico |
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Abstract: | By complementing a non-fermentative Escherichia coli (ldhA − pflB −) strain with the recombinant Zymomonas mobilis ethanol pathway (pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that there is a direct relationship between anaerobic growth rate and the total specific activity of pyruvate decarboxylase, which is the limiting enzyme of this specific fermentative NAD+ regenerating pathway. This relationship was proved to be useful to establish a selection strategy based on growth rate for the analysis of lctE libraries, which encode lactate dehydrogenase from Bacillus subtilis. |
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Keywords: | Growth rate control Lactate dehydrogenase NAD+ regeneration Non-fermentative Escherichia coli Pyruvate decarboxylase |
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