Monitoring CHO cell cultures: Cell stress and early apoptosis assessment by mass spectrometry |
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| Authors: | Sebastian Schwamb,Bogdan Munteanu,Bjö rn Meyer,Carsten Hopf,Mathias Hafner,Philipp Wiedemann |
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| Affiliation: | 1. Center for Applied Research in Biomedical Mass Spectrometry (ABIMAS), Paul-Wittsack-Str. 10, 68163 Mannheim, Germany;2. Mannheim University of Applied Sciences, Institute for Molecular and Cell Biology, Department of Biotechnology, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany;3. Mannheim University of Applied Sciences, Institute of Instrumental Analysis and Bioanalysis, Department of Biotechnology, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany;4. Institute of Medical Technology, Heidelberg University and Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany |
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| Abstract: | Mammalian cells, especially CHO (Chinese Hamster Ovary), are an important host for the production of biopharmaceuticals. Early detection of cellular stress and the onset of apoptosis, ultimately leading to a reduced viability of the culture, are important with respect to process development and monitoring. |
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| Keywords: | MALDI TOF MS Monitoring Cell culture Viability Apoptosis |
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