首页 | 本学科首页   官方微博 | 高级检索  
     


Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization
Authors:Sophie Trouvelot  Diederik van Tuinen  Mohamed Hijri  V. Gianinazzi-Pearson
Affiliation:(1) Laboratoire de Phytoparasitologie INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement, INRA, BV 1540, F-21034 Dijon Cedex, France e-mail : gianina@epoisses.inra.fr, Fax: +333-80-63-32-63, FR
Abstract: Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi. Accepted: 16 September 1998
Keywords:  Arbuscular mycorrhiza  Spore nuclei  Fluorescence in situ hybridization  Ribosomal DNA loci
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号