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Effects of various pretreatments on seed germination of<Emphasis Type="Italic">Calystegia soldanella</Emphasis> (Convolvulaceae), a coastal sand dune plant
Authors:Jung-Moon?Ko  Hye-Jeong?Park  Byeong-Mee?Min  Email author" target="_blank">Hyeon-Cheol?ChaEmail author
Institution:(1) Department of Biology, Dankook University, 330-714 Cheonan, Korea;(2) Department of Science Education, Dankook University, 140-714 Seoul, Korea
Abstract:We tested various pretreatments to enhance the germination of ‘Sea bells’ (Calystegia soldanella Roem. et Schult). One experiment, which improved the germination rate by 70%, involved first scarifying the seed coats, then immersing the seeds in 50 ppm GA3 for 24 h. A GA3-alone pretreatment did not increase germination. However, the most effective method, with a 100% success rate, included 3 h of acid pretreatment with 98% H2SO4. We also used scanning electron microscopy (SEM) to examine the seed-coat surfaces and cross-sections of dry seeds. SEM showed structural differences between seeds that were not treated and those exposed to 98% H2SO4. The latter treatment allowed the seed coats to crack and break, thereby disrupting their physical dormancy.
Keywords:Keyword" target="_blank">Keyword  Calystegia soldanella  GA3            H2SO4            scarification  seed germination  SEM
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