Genes encoding ribulosebisphosphate carboxylase and phosphoribulokinase are duplicated in Pseudomonas carboxydovorans and conserved in carboxydotrophic bacteria |
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Authors: | Iris Hugendieck Ortwin Meyer |
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Institution: | (1) Lehistuhl für Mikrobiologie der Universität Bayreuth, Universitätsstrasse 30, NWI, W-8580 Bayreuth, Federal Republic of Germany |
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Abstract: | Heterologous gene probes derived from cfxLp and cfxPp genes of Alcaligenes eutrophus H16 revealed the presence of structural genes encoding ribulosebisphosphate carboxylase (Rubisco) and phosphoribulokinase (PRK) on the genome of carboxydotrophic bacteria. The two genes were found to be rather conserved. In Pseudomonas carboxydovorans OM5 cfx genes reside on the plasmid pHCG3 and the chromosome as well, indicating that they are duplicated. Also in all plasmidharboring carboxydotrophic bacteria cfxL and cfxP structural genes were found to be plasmid-coded. Our results extend the list of carboxydotrophy structural genes residing on the plasmid pHCG3 and strongly support the idea that the components essential for the chemolithoautotrophic utilization of CO by Pseudomonas carboxydovorans OM5 are plasmid-coded. A cfxL gene probe from Rhodospirillum rubrum did not detectably hybridize with DNA from any of the carboxydotrophic bacteria examined.Abbreviations CODH
carbon monoxide dehydrogenase
- H2ase
hydrogenase
- kb
kilobase
- PRK
phosphoribulokinase
- Rubisco
ribulosebisphosphate carboxylase
- SDS
sodium dodecylsulfate |
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Keywords: | Carboxydotrophic bacteria Ribulosebis-phosphate carboxylase Phosphoribulokinase Hybridization Plasmids Genetics CO2 fixation Alcaligenes eutrophus Pseudomonas carboxydovorans Rhodospirillum rubrum |
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