The comparison between membrane and transorgan electric potentials inChenopodium rubrum: The methods |
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| Authors: | L. Adamec |
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| Affiliation: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, 16630 Praha 6, Czechoslovakia;(2) Present address: Institute of Botany, Section of Plant Ecology, Czechoslovak Academy of Sciences, Dukelská 145, 37982 Třeboň, Czechoslovakia |
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| Abstract: | A new method of simultaneous measurement of membrane (Em) and transorgan (Etr)electric potential in intactChenopodium rubrum plants and changes in Em and Etr under various experimental conditions are described. Em was measured in mesophyll leaf cells, and Etr in the same plant as a potential difference between a first pair leaf, tip and roots. The two potentials differed distinctly, Em averaging-156mV and Etr -5mV. But the,-changes in Em and Etr had approximately the same magnitude and time-course after changing light and darkness or KCl concentration in solution flowing around the leaf or during the photoperiodic induetive cycle. In relation to the same electrode connection they had an opposite polarity. The nature of Etr and its relation to Em are discussed. The measurement of Etr is much more stable and simpler than that of Em. The similarity of Em and Etr time-courses justifies the use of Etr measurement in electrophysiological studies as a replacement for Em measurements especially in intact plants. |
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