Flavodoxin overexpression reduces cadmium-induced damage in alfalfa root nodules |
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Authors: | Alla Shvaleva Teodoro Coba de la Peña Ana Rincón César N Morcillo Vanesa S García de la Torre M Mercedes Lucas José J Pueyo |
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Institution: | 1. Department of Plant Physiology and Ecology, Instituto de Recursos Naturales, Centro de Ciencias Medioambientales, CSIC, Serrano 115-bis, E-28006, Madrid, Spain 2. Lab Ecofisiologia Molecular, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av. da República, EAN, 2780-157, Oeiras, Portugal
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Abstract: | Flavodoxins are electron carrier flavoproteins that are involved in the response to oxidative stress in bacteria and cyanobacteria. Recently, we obtained Sinorhizobium meliloti bacteria that overexpressed a flavodoxin from the cyanobacterium Anabaena variabilis Redondo et al. (2009) Plant Physiology 149:1166–1178]. In the present work, tolerance to cadmium was evaluated in free-living transformed S. meliloti and in alfalfa plants nodulated by the flavodoxin-overexpressing rhizobia, in comparison with plants nodulated by wild-type bacteria. Overexpression of flavodoxin protected free-living S. meliloti from cadmium toxicity and had a positive effect on nitrogen fixation of alfalfa plants subjected to cadmium stress. Flavodoxin notably reduced cadmium-induced structural and ultrastructural alterations in alfalfa nodules. Putative protection mechanisms in flavodoxin-overexpressing nodules are discussed. Flavodoxin could have applications as a biotechnological tool to improve the symbiotic performance of alfalfa and other legumes in cadmium polluted soils. |
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