Purification and characterization of a chitosanase from Streptomyces N174 |
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Authors: | Isabelle Boucher Agnés Dupuy Pierre Vidal Witold A Neugebauer Ryszard Brzezinski |
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Institution: | (1) Groupe de Recherche en Biologie des Actinomycetes, Départment de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500, Boulevard Université, J1K 2R1 Sherbrooke, Québec, Canada;(2) Départment de Génie Chimique, Faculté des Sciences Appliquées, Université de Sherbrooke, 2500, Boulevard Université, J1K 2R1, Québec, Canada;(3) Division des Sciences Biologiques, Conseil National de Recherches du Canada, Ottawa, Ontario, Canada |
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Abstract: | A highly efficient chitosanase producer, the actinomycete N174, identified by chemotaxonomic methods as belonging to the genus Streptomyces was isolated from soil. Chitosanase production by N174 was inducible by chitosan or d-glucosamine. In culture filtrates the chitosanase accounted for 50–60% of total extracellular proteins. The chitosanase was purified by polyacrylic acid precipitation, CM-Sepharose and gel permeation chromatography. The maximum velocity of chitosan degradation was obtained at 65° C when the pH was maintained at 5.5. The enzyme degraded chitosans with a range of acetylation degrees from 1 to 60% but not chitin or CM-cellulose. The enzyme showed an endo-splitting type of activity and the end-product of chitosan degradation contained a mixture of dimers and trimers of d-glucosamine.Correspondence to: R. Brzezinski |
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