The localisation of enzymes of nitrogen assimilation in maize leaves and their activities during greening |
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| Authors: | E Harel P J Lea B J Miflin |
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| Institution: | (1) Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, U.K.;(2) Present address: Department of Botany, The Hebrew University, Jerusalem, Israel |
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| Abstract: | The activities of nitrate reductase (EC1.6.6.1), nitrite reductase (EC 1.6.6.4), glutamine synthetase (EC6.3.1.2), glutamate synthase (EC1.4.7.1) and NAD(P)H-dependent glutamate dehydrogenase (EC 1.4.1.3) were investigated in mesophyll and bundle sheath cells of maize leaves (Zea mays L.). Whereas nitrate and nitrite reductase appear to be restricted to the mesophyll and GDH to the bundle sheath, glutamine synthetase and glutamate synthase are active in both tissues.During the greening process, the activities of nitrate and nitrite reductase increased markedly, but glutamine synthetase, glutamate synthase and glutamate dehydrogenase changed little.Abbreviations BDH
British Drug Houses
- EDTA
Ethylene diamine tetra-acetic acid
- GDH
Glutamate dehydrogenase
- NADH
Nicotinamide-adenine dinucleotide reduced form
- NADPH
Nicotnamide-adenine dinucleotide phosphate reduced form
- PMSF
Phenylmethyl sulphonyl fluoride |
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| Keywords: | Enzyme localisation Glutamate synthase Glutamine synthetase Greening Nitrogen metabolism Zea mays |
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