Physical arrangement of suppressor-sensitive mutations of Bacillus phage M2 |
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Authors: | Kouji Matsumoto and Hideo Hirokawa |
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Institution: | (1) Life Science Institute, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, 102 Tokyo, Japan |
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Abstract: | Summary The order of the fragments derived from bacteriophage M2 DNA by digesting it with restriction endonucleases Xba1, HindIII, and EcoRI has been determined. The locus of each representative mutation in 13 cistrons of the M2 genome has been determined by transfection/marker rescue with the individual restriction enzyme-digested fragments derived from wild-type M2 DNA. |
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