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Somatic embryogenesis and adventitious shoot formation in Burma reed (<Emphasis Type="Italic">Neyraudia arundinacea</Emphasis> Henr.)
Authors:Guohua Ma  Guojiang Wu  Eric Bunn
Institution:(1) South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China;(2) Kings Park and Botanic Garden, West Perth, Western Australia, 6005, Australia
Abstract:Burma reed (Neyraudia arundinacea Henr.) is a C4 grass native to Southeast Asia and Indomalaya that grows quickly, exhibits strong resistance to environmental stresses, and is extremely adaptable. It can be widely utilized as a bioenergy crop for biomass conversion. In vitro multiple shoots were first established from axillary buds and then subcultured on propagation medium containing 10 μM 6-benzylaminopurine (BA) and 2.0 μM naphthaleneacetic acid (NAA). Multishoot clumps were used as explants to induce somatic embryogenesis and adventitious shoot formation. The results showed that auxin 2,4-dichlorophenoxyacetic acid or NAA play a key role for the induction of somatic embryogenesis and adventitious shoot formation, whereas cytokinin BA or kineatin enhance shoot proliferation and plant regeneration from callus and somatic embryos. Efficient somatic embryogenesis, mass propagation, and plant regeneration systems in Burma reed were established.
Keywords:Neyraudia arundinacea Henr    Somatic embryogenesis  Adventitious shoot formation  Mass propagation  Plant regeneration
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