Somatic embryogenesis and adventitious shoot formation in Burma reed (<Emphasis Type="Italic">Neyraudia arundinacea</Emphasis> Henr.) |
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Authors: | Guohua Ma Guojiang Wu Eric Bunn |
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Institution: | (1) South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China;(2) Kings Park and Botanic Garden, West Perth, Western Australia, 6005, Australia |
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Abstract: | Burma reed (Neyraudia arundinacea Henr.) is a C4 grass native to Southeast Asia and Indomalaya that grows quickly, exhibits strong resistance to environmental stresses, and
is extremely adaptable. It can be widely utilized as a bioenergy crop for biomass conversion. In vitro multiple shoots were
first established from axillary buds and then subcultured on propagation medium containing 10 μM 6-benzylaminopurine (BA)
and 2.0 μM naphthaleneacetic acid (NAA). Multishoot clumps were used as explants to induce somatic embryogenesis and adventitious
shoot formation. The results showed that auxin 2,4-dichlorophenoxyacetic acid or NAA play a key role for the induction of
somatic embryogenesis and adventitious shoot formation, whereas cytokinin BA or kineatin enhance shoot proliferation and plant
regeneration from callus and somatic embryos. Efficient somatic embryogenesis, mass propagation, and plant regeneration systems
in Burma reed were established. |
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Keywords: | Neyraudia arundinacea Henr Somatic embryogenesis Adventitious shoot formation Mass propagation Plant regeneration |
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