Intertribal somatic hybrids between <Emphasis Type="Italic">Brassica napus</Emphasis> and <Emphasis Type="Italic">Camelina sativa</Emphasis> with high linolenic acid content |
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| Authors: | J J Jiang X X Zhao W Tian T B Li Y P Wang |
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| Institution: | (1) College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China;(2) Biotechnology Key Laboratory of Eco-Agriculture in Hongze Lake Region, Huaiyin Normal College, Huaian, 223001, China; |
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| Abstract: | Intertribal somatic hybrids of Brassica napus and Camelina sativa were developed by protoplast electrofusion. Hybrid identity of the regenerants was determined using flow cytometric analysis
of nuclear DNA content and simple sequence repeat (SSR) marker analysis. Three hybrids exhibited specific bands for B. napus and C. sativa. These hybrids showed intermediate leaf, flower and seed morphology compared with the two parental species. The seeds of
these three hybrids had a modified fatty acid profile, indicating higher level of linolenic and eicosanoic acids than those
of B. napus. Our results suggest that somatic hybridization offers opportunities for transferring entire genomes between B. napus and C. sativa in improving rapeseed breeding. |
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