Effect of local secondary structure of mRNA on expression of recombinant human lymphotoxin inEscherichia coli |
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| Authors: | Seungkiel Park Moonhee Sung Hyangsook Yoo M. Y. Pack |
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| Affiliation: | (1) Department of Biotechnology, KAIST, 305-701 Daejon, Korea;(2) Genetic Engineering Research Institute, KIST, P.O. Box 17, 305-606 Daedok Science Town, Daejon, Korea |
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| Abstract: | We substituted a few alternative codons close to the N-terminal coding region of human lymphotoxin (LT) gene for natural ones to avoid stable local secondary structures of mRNA, predicted in the 5 -terminal 130 nucleotides of mRNA. With this modification, we have overexpressed a recombinant lymphotoxin (rLT) inEscherichia coli under the control of trc promoter. Most of the produced rLT was a soluble and active form. |
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