球囊霉属几种AM真菌孢子表面消毒与萌发的研究*

通过对球囊霉属几种AM真菌进行孢子表面消毒与萌发研究,结果表明,采用“差别灭菌法”即先用含2%氯胺T、0.02%链霉素和0.01%庆大霉素的溶液孢子表面消毒10min,然后在25~27℃下培养3天,再用含2%氯胺T、0.02%链霉素和0.01%庆大霉素的溶液孢子表面消毒10min,其消毒效果最好,孢子萌发率较高,污染率较低。同时发现土壤、草炭和寄主植物根的分泌物对孢子萌发有促进作用。培养基pH值的变化对孢子萌发也有一定的影响。AM真菌孢子用“改良Sandwich法”进行萌发,其萌发率最高。

THE STUDY FOR SURFACE-STERILIZATION AND GERMINATION OF GLOMUS SPORES

In this paper, the study on surface~sterilization and germination of Glomus spores was carried out. The results showed that the sterilization effect in 搕he differential method of sterilization?adopted was the best. Namely spores firstly was sterilized for 10 min in a solution of 2% chloramine T、 0.02%streptomycin and 0.01% gentamycin. After that spores germinated for 3 days under 25~27℃ and then was sterilized for 10 min in a solution of 2% chloramine T、0.02% streptomycin and 0.01%gentamycin. Seen from the results, the germinant rate of spores was higher and the contamination rate was much lower. At the same time, we found that Soils, peat and secretory products of plant roots could accelerate the germination of spores. Different pH has some influence on the germination of spores. Moreover, the germinant rate of spores was the highest in 搕he method of the improved sandwich?