Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence |
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Authors: | Ulrich Plagens Arno L. Greenleaf Ekkehard K. F. Bautz |
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Affiliation: | (1) European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg;(2) Molekulare Genetik der Universität, 6900 Heidelberg, West Germany |
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Abstract: | Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock. |
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