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Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence
Authors:Ulrich Plagens  Arno L Greenleaf  Ekkehard K F Bautz
Institution:(1) European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg;(2) Molekulare Genetik der Universität, 6900 Heidelberg, West Germany
Abstract:Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock.
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