| 转基因植物中外源基因的整合特性及其研究策略 |
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| 引用本文： | 曾凡锁,詹亚光.转基因植物中外源基因的整合特性及其研究策略[J].植物学报,2004,21(5):565-577. |
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| 作者姓名： | 曾凡锁 詹亚光 |
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| 作者单位： | (东北林业大学生命科学学院 哈尔滨 150040) |
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| 摘 要： | 综述了外源基因在宿主中的整合特性及结构变化的多样性。将外源基因在宿主中的整合方式分为3种：单拷贝单位点整合、串联多联体单位点整合及单拷贝或多拷贝在多位点整合。分析了不同整合方式对表达的影响。详细讨论了植物转基因的整合机制，阐述了以基因组的双链断裂修复为基础的整合模型。最后介绍了国内外的研究者对不同的整合方式所采取的研究策略。
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| 关 键 词： | 转基因植物 整合 表达 外源基因 |
| 收稿时间： | 2003-11-25 |
| 修稿时间： | 2004-01-12 |
Integration of Exogenous Genes in Transgenic Plant Genomes: Characteristics and Approaches |
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| ZENG Fan-Suo ZHAN Ya-Guang.Integration of Exogenous Genes in Transgenic Plant Genomes: Characteristics and Approaches[J].Bulletin of Botany,2004,21(5):565-577. |
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| Authors: | ZENG Fan-Suo ZHAN Ya-Guang |
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| Institution: | (College of Life Sciences, Northeast Forestry University, Harbin 150040) |
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| Abstract: | The integration of exogenous genes in transgenic plant genome are reviewed. Three integration patterns of exogenous genes included single-copy integration in one site, multiple-copy integration in one site and single-copy or multiple-copy integration in multiple sites. This review also analyses the influence of different integration patterns on the expression of exogenous genes, and discusses the mechanism of integration and the T-DNA integration model based on genomic DSB (double-strand breaks). In the end, the approaches studying different pattern of integration are introduced. |
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| Keywords: | , |
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