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| 双歧杆菌质粒聚合酶基因对质粒载体稳定性的影响 | |
| 引用本文： | 荀安营,王立生,李娜,朱忠生,李迎雪,龙若庭,曾位森,朱惠明.双歧杆菌质粒聚合酶基因对质粒载体稳定性的影响[J].中国微生态学杂志,2009,21(2):104-105. |
| 作者姓名： | 荀安营 王立生 李娜 朱忠生 李迎雪 龙若庭 曾位森 朱惠明 |
| 作者单位： | 1. 暨南大学第二临床医学院,消化科,广东,深圳,518020 2. 暨南大学第二临床医学院,呼吸科,广东 3. 中国医科大学深圳市儿童医院,内科,广东,深圳,518026 4. 南方医科大学,细胞生物学教研室,广东,广州,510515 |
| 摘 要： | 目的探讨双歧杆菌天然质粒的聚合酶基因（Bifidobacterium plasmid polymerase，BPP）对人工构建的大肠埃希菌～双歧杆菌穿梭质粒载体（shuttle vector）在长双歧杆菌中稳定性的影响。方法首先电转化质粒pBADs—A和pBADs-BPP至长双歧杆菌，培养鉴定后，接种含质粒pBADs—A和pBADs-BPP的双歧杆菌于AMP^-和AMP^＋的MRS培养液中。厌氧培养后，将样品涂布于AMP^＋的MRS固体培养板上计数菌落数。结果相同条件下质粒pBADs·BPP组菌落数高于质粒pBADs—A组（P〈0．05）。结论BPP可以增加质粒载体的稳定性。 |
| 关 键 词： | 双歧杆菌 质粒聚合酶基因 质粒载体 稳定性 |
Influence of Bifidobacterium plasmid polymerase on stability of plasmid vector |
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| XUN An-ying,WANG Li-sheng,LI Na,ZHU Zhong-sheng,LI Ying-xue,LONG Ruo-ting,ZENG Wei-sen,ZHU Hui-ming.Influence of Bifidobacterium plasmid polymerase on stability of plasmid vector[J].Chinese Journal of Microecology,2009,21(2):104-105. | |
| Authors: | XUN An-ying WANG Li-sheng LI Na ZHU Zhong-sheng LI Ying-xue LONG Ruo-ting ZENG Wei-sen ZHU Hui-ming |
| Institution: | XUN An-ying , WANG Li-sheng, LI Na, ZHU Zhong-sheng, LI Ying-xue , LONG Ruo-ting, ZENG Wei-sen ,ZHU Hui-ming (1. Department of Gastroenterology,the Second Clinical Medical Callege , Jinan University , Shenzhen 518020, China;2. Department of Respirary , the Second Clinical Medical College, J inan University, Shenzhen 518020, China; 3. Sheazhen Children＇s Hospital of China Medical University, Shenzhen 518026, China; 4. Department of Cytobiology , Southern Medical University, Guangzhou 510515, China) |
| Abstract: | Objective To explore the influence of Bifidobacterium plasmid polymerase （BPP） on stability of plasmid vector in Bifidobacterium longum. Method The plasmid vector pBADs-A and pBADs-BPP were electrotransformed into Bifidobacterium longum. The positive colonies were screened and identified. The equivalent strains were inoculated in AMP^- and AMP^＋ MRS media and cultured in anaerobiotic environment. The culture solution was then diluted and spread on AMP^＋ MRS medium plates, and the number of colony in plates was counted. Result Under the same condition, the number of colony of plasmid pBADs-BPP group was more than that of plasmid pBADs-A group （ P 〈 0.05 ）. Conclusion BPP can increase the stability of plasmid vector in Bifidobacterium Iongum. |
| Keywords: | Bifutobacterium Bifutobacterium plasmid polymerase Plasmid vector Stability |
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