Identification of the thrombin light chain a as the single best mass for differentiation of gastric cancer patients from individuals with dyspepsia by proteome analysis |
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Authors: | Ebert Matthias P A Lamer Stephanie Meuer Jörn Malfertheiner Peter Reymond Marc Buschmann Thomas Röcken Christoph Seibert Volker |
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Affiliation: | Department of Gastroenterology, General Surgery and Pathology, Otto-von-Guericke University, D-39120 Magdeburg, Germany. Matthias.Ebert@medizin.uni-magdeburg.de |
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Abstract: | Gastric cancer mortality is second only to lung cancer, and its prognosis is dismal. Using surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry, we previously identified a single best mass, which could separate gastric cancer from patients without cancer, with a sensitivity of 89.9% and a specificity of 90%. Using protein liquid chromatography systems with various chromatography media and MS/MS analysis, we were able to identify thrombin light chain A, a proteolytic fragment of prothrombin, as the single best mass for early detection of gastric cancer patients. These findings indicate that disturbances in the coagulation-system are early events in gastric cancer biology and that a decrease or loss of thrombin light chain A, which we termed negative serum protein profiling, may contribute to the diagnosis of cancer patients. |
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