A Study of Glutathione <Emphasis Type="Italic">S</Emphasis>-transferase pi Expression in Central Nervous System of Subjects with Amyotrophic Lateral Sclerosis Using RNA Extraction from Formalin-Fixed,Paraffin-Embedded Material |
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Authors: | Ewa?Usarek Beata?Gajewska Beata?Ka?mierczak Magdalena?Ku?ma Dorota?Dziewulska Email author" target="_blank">Anna?Barańczyk-Ku?maEmail author |
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Institution: | (1) Chair and Department of Biochemistry, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland;(2) Department of Neurology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland |
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Abstract: | The expression of glutathione S-transferase pi (GST pi), an enzyme responsible for inactivation of a large variety of toxic compounds was studied in spinal
cord, motor and sensory brain cortex obtained from patients who died in the course of amyotrophic lateral sclerosis (ALS).
The studies were performed on formalin-fixed, paraffin-embedded (FFPE) and freshly frozen tissues. The method of RNA isolation
from FFPE was modified. A significant decrease of GST pi-mRNA expression was found in cervical spinal cord and motor brain
cortex of ALS subjects comparing to analogue control tissues (P < 0.01), as well as in motor cortex of ALS subjects comparing to their sensory cortex (P < 0.05). In spinal cords the decrease in GST pi-mRNA expression was accompanied by a decrease of GST pi protein level. Results
indicated lowered GST pi expression on both mRNA and protein levels in the regions of nervous system affected by ALS. The
non-properly inactivated by GST toxic electrophiles and organic peroxides may thus contribute to motor neurons damage. |
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Keywords: | Amyotrophic lateral sclerosis brain cortex formalin-fixed Glutathione S-transferase pi paraffin-embedded material spinal cord |
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