Quantifying heterogeneity: flow cytometry of bacterial cultures |
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| Authors: | Douglas B. Kell Hazel M. Ryder Arseny S. Kaprelyants Hans V. Westerhoff |
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| Affiliation: | (1) Department of Biological Sciences, University College of Wales, SY23 3DA Aberystwyth, Dyfed, UK;(2) Bakh Institute of Biochemistry, USSR Academy of Sciences, Leninskii Prospekt 33, 117071 Moscow, USSR;(3) Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands;(4) E. C. Slater Institute for Biochemical Research, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands |
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| Abstract: | Flow cytometry is a technique which permits the characterisation of individual cells in populations, in terms of distributions in their properties such as DNA content, protein content, viability, enzyme activities and so on. We review the technique, and some of its recent applications to microbiological problems. It is concluded that cellular heterogeneity, in both batch and continuous axenic cultures, is far greater than is normally assumed. This has important implications for the quantitative analysis of microbial processes. |
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| Keywords: | analysis bacteria cytofluorometry flow cytometry heterogeneity |
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