Sodium binding to and protonation of ATP: a multinuclear magnetic double resonance study at 8.46 tesla |
| |
| Authors: | U Pilatus A Mayer W Offermann D Leibfritz |
| |
| Institution: | Fachbereiche Physik, Universit?t Bremen, F.R.G. |
| |
| Abstract: | We show that the interaction of ATP with Na+ and H+, whether binding or dissociation, gives rise to exchange broadened 31P-NMR spectra at 8.4 T, pH 6.7 and 310 K. We interpret the effect as being due to a two-step conversion between two NMR-differentiated ATP pools. A quantitative analysis yields all involved equilibrium constants and some of the dynamic parameters. Our results help to understand previous studies of magnesium binding to ATP and the appearance of high-field in vivo 31P-NMR spectra. |
| |
| Keywords: | |
|
|
