Responses of BrdU label-retaining dental pulp cells to allogenic tooth transplantation into mouse maxilla |
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Authors: | Noriko Mutoh Mitsushiro Nakatomi Hiroko Ida-Yonemochi Eizo Nakagawa Nobuyuki Tani-Ishii Hayato Ohshima |
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Institution: | (1) Division of Endodontics, Department of Oral Medicine, Kanagawa Dental College, Yokosuka, Japan;(2) Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan;(3) Division in Anatomy and Developmental Biology, Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea; |
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Abstract: | Recently, we demonstrated that a pulse of BrdU given to prenatal animals reveals the existence of slow-cycling long-term label-retaining
cells (LRCs), putative adult stem or progenitor cells, which reside in the dental pulp. This study aims to clarify responses
of LRCs to allogenic tooth transplantation into mouse maxilla using prenatal BrdU-labeling, in situ hybridization for osteopontin and periostin, and immunohistochemistry for BrdU, nestin, and osteopontin. The upper-right first molars were allografted in the original
socket between BrdU-labeled and non-labeled mice or between GFP transgenic and wild-type mice. Tooth transplantation caused
degeneration of the odontoblast layer, resulting in the disappearance of nestin-positive reactions in the dental pulp. On
postoperative days 5–7, tertiary dentin formation commenced next to the preexisting dentin where nestin-positive odontoblast-like
cells were arranged in the successful cases. In BrdU-labeled transplanted teeth, dense LRCs were maintained in the center
of the dental pulp beneath the odontoblast-like cells including LRCs, whereas LRCs disappeared in the area surrounding the
bone-like tissue. In contrast, LRCs were not recognized in the pulp chamber of non-labeled transplants through the experimental
period. Tooth transplantation using GFP mice demonstrated that the donor cells constituted the dental pulp of the transplant
except for endothelial cells and some migrated cells, and the periodontal tissue was replaced by host-derived cells except
for epithelial cell rests of Malassez. These results suggest that the maintenance of BrdU label-retaining dental pulp cells
play a role in the regeneration of odontoblast-like cells in the process of pulpal healing following tooth transplantation. |
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