| 灰树花多糖的提取及抗氧化活性 |
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| 引用本文： | 顾华杰,沈晨斌,严志舟,金琎,王桃云,胡翠英,陈家长.灰树花多糖的提取及抗氧化活性[J].生物加工过程,2012,10(1):19-24. |
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| 作者姓名： | 顾华杰 沈晨斌 严志舟 金琎 王桃云 胡翠英 陈家长 |
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| 作者单位： | 1. 苏州科技学院化学与生物工程学院,苏州,215009
2. 中国水产科学研究院淡水渔业研究中心,无锡,214081 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助项目，苏州科技学院基金资助项目 |
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| 摘 要： | 采用单因素和正交试验法对热水浸提、超声波辅助和微波辅助提取灰树花多糖的工艺进行研究,分别获得了3种方法的最佳条件,并发现微波辅助法最佳,其最优条件为:微波功率800 W、微波时间3 min,浸提2h,多糖得率为13.05％,比热水浸提法提高了51.22％.利用提取的灰树花多糖进行抗氧化性研究,发现灰树花多糖对O2-·和·OH都具有一定的清除作用,且多糖浓度与其对O2-·的清除作用存在量效关系,但不如·OH明显.
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| 关 键 词： | 灰树花 多糖 提取 抗氧化能力 |
Extraction of Grifola frondosa polysaccharides and test of their antioxidative activities |
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| GU Huajie,SHEN Chenbin,YAN Zhizhou,JIN Jin,WANG Taoyun,HU Cuiying,CHEN Jiachang.Extraction of Grifola frondosa polysaccharides and test of their antioxidative activities[J].Chinese Journal of Bioprocess Engineering,2012,10(1):19-24. |
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| Authors: | GU Huajie SHEN Chenbin YAN Zhizhou JIN Jin WANG Taoyun HU Cuiying CHEN Jiachang |
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| Institution: | 1.College of Chemical and Biological Engineering,Suzhou University of Science and Technology,Suzhou 215009,China; 2.Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China) |
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| Abstract: | Single factor and orthogonal tests were used to study three extraction techniques of Griflola frondosas polysaccharides,i.e.,hot water method,ultrasonic-assisted extraction method and microwave-assisted extraction method.The optimal conditions of three method were obtained： optimal conditions were 800 W of microwave power,3 min of microwave time,2 h of heating time,the yield was 13.05%.It increased 51.22%,compared with the hot water method.The yield of polysaccharides by the microwave-assisted extraction was the most.The antioxidative activities of the polysaccharides were analyzed.Results indicated that Griflola frondosas polysaccharides had peroxide radical and hydroxyl radical scavenging capabilities. |
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| Keywords: | Griflola frondosa polysaccharides extraction antioxidant |
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