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螺旋藻转化纳米元素硒的制备及其体外清除自由基活性的初步研究
引用本文:靳兴媛,周永林,任璐艳,张逸波,凌钦婕,黄峙. 螺旋藻转化纳米元素硒的制备及其体外清除自由基活性的初步研究[J]. 中国生物工程杂志, 2012, 32(1): 30-35
作者姓名:靳兴媛  周永林  任璐艳  张逸波  凌钦婕  黄峙
作者单位:暨南大学生命科学技术学院 广州 510632
摘    要:研究利用高密度富硒螺旋藻(Se-SP)细胞通过生物转化制备纳米元素硒(Nano-Se)的可行性,观察Nano-Se在体外对氧自由基的清除作用。用梯度离心分选Nano-Se,原子力显微镜(AFM)、透射电镜(TEM)及X-射线能谱(EDX)联用表征纳米粒中的元素硒形态,电感耦合等离子质谱仪(ICP-MS)测定Nano-Se中的硒含量,化学发光方法检测Nano-Se在体外对超氧自由基和羟自由基的清除作用。结果发现,Nano-Se主要由元素硒构成,形态呈球形,73%的纳米粒子直径大小分布在(61±17)nm范围。Nano-Se在体外对两种氧自由基的最大清除率分别为:30.1%和27.6%,相应的EC50分别为:0.8 μg/ml和2.2 μg/ml。相同剂量时,Nano-Se对氧自由基的清除作用比硒代蛋氨酸(Se-Met)及Se-SP中其它含硒活性成分更强。结果提示,利用高密度Se-SP可诱导Nano-Se的大量生成,Se-SP转化的Nano-Se可能是一种新的抗氧化硒形态,其作用机制和体内生物活性有待深入研究。

关 键 词:纳米元素硒  螺旋藻  自由基  
收稿时间:2011-09-14
修稿时间:2011-10-26

Nano Elemental Selenium Bio-transformed from S.platensis and Scavenging Activity on Oxygen Free Radicals in vitro
JIN Xing-yuan,ZHOU Yong-lin,REN Lu-yan,ZHANG Yi-bo,LING Qin-jie,HUANG Zhi. Nano Elemental Selenium Bio-transformed from S.platensis and Scavenging Activity on Oxygen Free Radicals in vitro[J]. China Biotechnology, 2012, 32(1): 30-35
Authors:JIN Xing-yuan  ZHOU Yong-lin  REN Lu-yan  ZHANG Yi-bo  LING Qin-jie  HUANG Zhi
Affiliation:(Department of Biotechnology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China)
Abstract:Preparation of Nano elemental selenium(Nano-Se) bio-transformed from Se enriched S.platensis(Se-SP) and scavenging activity on oxygen free redicals was investigated.Nano-Se was harvested from high cells density cultures of Se-SP with total Se supplementation of 600 μg/ml in form of sodium selenite.The shape and size of Nano-Se was characterized by atomic force microscope(AFM),transmission electron microcope(TEM) and energy-dispersive X-ray(EDX).Se contents were detected by inductively coupled plasma mass spectrometry(ICP-MS).The scavenging activities of Nano-Se on superoxide anions and hydroxyl radicals were detected by chemiluminescence method.The data showed that the bio-transformed Nano-Se was constructed mainly by elemental Se.A 73% fraction of Nano-Se was collected by gradient centrifugation,in which it was spherical in shape and uniform in size with average diameter of(61±17)nm.In vitro maximum scavenging rates of Nano-Se on superoxide anions and hydroxyl radicals were 30.1% and 27.6%,and the correspondence EC50 were 0.8 and 2.2 μg/ml,respectively.The scavenging activities of Nano-Se on oxygen free radicals were much higher than that of selenomethinoine and other Se containing compounds isolated from Se-SP at the same dosages.In conclusion,present results suggested that Nano-Se produced by high cells density cultures of Se-SP is a novel Se species with anti-oxidative activity in vitro.
Keywords:Nano elemental selenium(Nano-Se) Spirulina platensis Free radical
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