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Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments
Authors:T Cremer  C Cremer  T Schneider  H Baumann  L Hens  M Kirsch-Volders
Institution:(1) Institut für Anthropologie und Humangenetik, University of Heidelberg, Im Neuenheimer Feld 328, D-6900 Heidelberg, Federal Republic of Germany;(2) Institut für Humangenetik und Anthropologie, University of Freiburg i. Br., Albertstr. 11, D-7800 Freiburg, Federal Republic of Germany;(3) Department of Human Genetics, Free University of Brussels (V.U.B.), Pleinlaan 2, B-1050 Brussels, Belgium
Abstract:Summary Unsynchronized cells of an essentially diploid strain of female Chinese hamster cells derived from lung tissue (CHL) were laser-UV-microirradiated (lambda=257 nm) in the nucleus either at its central part or at its periphery. After 7–9 h postincubation with 0.5 mM caffeine, chromosome preparations were made in situ. Twenty-one and 29 metaphase spreads, respectively, with partial chromosome shattering (PCS) obtained after micro-irradiation at these two nuclear sites, were Q-banded and analyzed in detail. A positive correlation was observed between the frequency of damage of chromosomes and both their DNA content and length at metaphase. No significant difference was observed between the frequencies of damage obtained for individual chromosomes at either site of microirradiation. The frequency of joint damage of homologous chromosomes was low as compared to nonhomologous ones. Considerable variation was noted in different cells in the combinations of jointly shattered chromosomes. Evidence which justifies an interpretation of these data in terms of an interphase arrangement of chromosome territories is discussed. Our data strongly argue against somatic pairing as a regular event, and suggest a considerable variability of chromosome positions in different nuclei. However, present data do not exclude the possibility of certain non-random chromosomal arrangements in CHL-nuclei. The interphase chromosome distribution revealed by these experiments is compared with centromere-centromere, centromere-center and angle analyses of metaphase spreads and the relationship between interphase and metaphase arrangements of chromosomes is discussed.
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