Identification of cDNAs encoding pterocarpan reductase involved in isoflavan phytoalexin biosynthesis in Lotus japonicus by EST mining |
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Authors: | Akashi Tomoyoshi Koshimizu Shoko Aoki Toshio Ayabe Shin-Ichi |
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Institution: | Department of Applied Biological Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. akashi@brs.nihon-u.ac.jp |
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Abstract: | Isoflavans and pterocarpans are the major biosynthetically connected phytoalexins in legumes. A search of the expressed sequence tag library of a model legume Lotus japonicus, which produces an (-)-isoflavan, for homologs of phenylcoumaran benzylic ether reductase catalyzing the reductive cleavage of dihydrofurans, yielded seven full-length cDNAs, and the encoded proteins were analyzed in vitro. Four of them cleaved the dihydrofuran of a pterocarpan medicarpin to yield an isoflavan (-)-vestitol and were designated pterocarpan reductase (PTR). Two PTRs displayed enantiospecificity to (-)-medicarpin, representing genuine L. japonicus PTRs, while the other two lacked enantiospecificity and were presumed to be evolutionarily primitive types. |
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Keywords: | DMID 7 2′-dihydroxy-4′-methoxyisoflavanol dehydratase EST expressed sequence tag GSH reduced glutathione IFR isoflavone reductase NMR nuclear magnetic resonance PCBER phenylcoumaran benzylic ether reductase PLR pinoresinol-lariciresinol reductase PTR pterocarpan reductase Rt retention time SDR short-chain dehydrogenase/reductase VR vestitone reductase |
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