Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) beta-1,3-glucanase gene |
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Authors: | Yu Zeng Ying Xu Lin Tang Xiao-Lin Qiang Fang Chen |
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Affiliation: | (1) College of Life Science, Sichuan University, Chengdu, 610064, China;(2) Tibet Agriculture Research Institute, Tibet, 850000, China |
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Abstract: | A new full-length -1,3-glucanase cDNA was obtained by RT-PCR and RACE techniques from Tibet hulless barley and its complete gene sequence obtained by DNA Walking. Sequence alignment with the BLAST program showed that cDNA has high similarity with barley -1,3-glucanase II. The gene was functionally expressed in E. coli and the recombinant protein catalysed the hydrolysis of -1,3-glucan with an action pattern characteristic of a -1,3-glucan endohydrolase (EC 3.2.1.39). Southern blot analysis indicated that the gene is a member of a small gene family. RT-PCR and northern blot analysis indicated it is constitutively expressed in barley shoots. |
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Keywords: | /content/u422r67023p47184/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-1,3-glucanase gene hulless barley pathogenesis-related transform |
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