Age related differences in the plasma kinetics of flavanols in rats |
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Affiliation: | 1. Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University, Spain;2. Technological Center of Nutrition and Health (CTNS), TECNIO, CEICS, Reus, Spain;1. Department of Pediatric Cardiology, Ghent University Hospital, Ghent, Belgium;2. Department of Anesthesiology, Ghent University Hospital, Ghent, Belgium;3. Department of Cardiac Surgery, Ghent University Hospital, Ghent, Belgium;4. Department of Movement and Sports Sciences, Ghent University, Ghent, Belgium;1. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, Jiangsu 211816, China;2. College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China;3. College of Biological and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China |
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Abstract: | Dietary flavanols produce beneficial health effects; once absorbed, they are recognized as xenobiotics and undergo Phase-II enzymatic detoxification. However, flavanols with a degree of polymerization greater than 2 reach the colon, where they are subjected to microbial metabolism and can be further absorbed and undergo Phase-II reactions. In this sense, flavanols' health-promoting properties are mainly attributed to their metabolic products. Several age-related physiological changes have been evidenced, and it is known that flavanols' bioavailability is affected by internal factors. Therefore, this study aimed to elucidate whether animals of different ages, specifically young and adult rats, exhibit differences in their flavanol metabolism and plasma bioavailability. To accomplish this, an acute dose of a grape seed polyphenol extract was administered to male rats; after 2, 4, 7, 24 and 48 h, flavanols and their Phase-II and microbial metabolites were quantified by HPLC-ESI-MS/MS in plasma. The results indicated important age-related quantitative differences in plasma flavanol metabolites. Interestingly, adult rats presented a remarkable reduction in flavanol absorption and Phase-II flavanol metabolism. Consequently, microbial-derived flavanol metabolism is triggered by higher flavanol affluence in the colonic tract. Furthermore, young rats presented a faster metabolic profile than adult rats. Hence, our results indicate that the physiological bioactivities of flavanols may depend on age. |
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