Operationally defined species characterization and bioaccessibility evaluation of cobalt,copper and selenium in Cape gooseberry (Physalis Peruviana L.) by SEC-ICP MS |
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| Affiliation: | 1. Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Beijing, China;2. College of Resource Science & Technology, Beijing Normal University, Beijing, China;3. College of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China;1. Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Berdychowo 4, 60-965 Poznań, Poland;2. Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland;1. Institute of Pomology, Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng), Ministry of Agriculture, Xingcheng 125100, P.R. China;2. Yantai Academy of Agricultural Sciences, Yantai 265500, P.R. China;3. Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumchi 830091, P.R. China;4. Institute of Tianjin Agriculture Quality Standard and Testing Technology, Tianjin 300381, P.R. China;5. Beijing Research Center for Agricultural Standards and Testing, Beijing 100097, P.R. China;6. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, P.R. China;7. Zhengzhou Institute of Pomology, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R. China;1. Institute of Nutrition and Food Science (INFS), University of Dhaka, Dhaka 1000, Bangladesh;2. Department of Soil Science, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh;3. Department of Oceanography, University of Dhaka, Dhaka 1000, Bangladesh;1. Department of Food Technology, Engineering and Nutrition, Lund University, P.O. Box 124, 22100 Lund, Sweden;2. Food and Natural Products Center, San Simon University, Cochabamba, Bolivia;3. Department of Food Science, Chalmers University of Technology, S-40229 Göteborg, Sweden |
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| Abstract: | Physalis peruviana could attract great interest because of its nutritional and industrial properties. It is an excellent source of vitamins, minerals, essential fatty acids and carotenoids. Physalis Peruviana is also known to have a positive impact on human health. Unfortunately, still little is known about trace elements present in Physalis Peruviana and their forms available for the human body. Thus, the aim of this study was to estimate bioaccessibility and characterization of species of cobalt, copper and selenium in Physalis Peruviana fruits.Total and extractable contents of elements were determined by mass spectrometer with inductively coupled plasma (ICP MS). In order to separate the different types of metal complexes Physalis peruviana fruits were treated with the following solvents: Tris–HCl (pH 7.4), sodium dodecyl sulfate (SDS) (pH 7.4) and ammonium acetate (pH 5.5). The best efficiency of extraction of: cobalt was obtained for ammonium acetate (56%) and Tris–HCl (60%); for copper was obtained for SDS (66%), for selenium the best extraction efficiency was obtained after extraction with SDS (48%).To obtain information about bioaccessibility of investigated elements, enzymatic extraction based on in vitro simulation of gastric (pepsin) and intestinal (pancreatin) digestion was performed. For copper and selenium the simulation of gastric digestion leads to the extraction yield above 90%, while both steps of digestion method were necessary to obtain satisfactory extraction yield in the case of cobalt.Size exclusion chromatography (SEC) coupled to on-line ICP MS detection was used to investigate collected metal species. The main fraction of metal compounds was found in the 17 kDa region. Cobalt and copper create complexes mostly with compounds extracted by means of ammonium acetate and SDS, respectively. Cobalt, copper and selenium were found to be highly bioaccessible from Physalis Peruviana. Investigation of available standards of cobalt and selenium allows confirming the presence of vitamin B12 and probably selenomethionine in the fraction bioaccessible by human body (obtained during enzymatic extraction). It should be noted that the presence of small seleno-compounds in Cape gooseberry was performed for the first time.The results show that the combination of SEC and ICP MS could provide a simple method for separating of soluble element species. |
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| Keywords: | Bioaccessibility SEC-ICP MS Selenomethionine |
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