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Synthesis,characterization, and wound healing properties of α/γ hybrid peptides |
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| Authors: | Junaid ur Rahim Arfan Khalid Sudha Shankar Irfan Qasam Govind Yadav Rajkishor Rai |
| Institution: | 1. Natural Products and Medicinal Chemistry Division (NPMC), CSIR-Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir, India
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India;2. Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir, India Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India;3. Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir, India;4. Natural Products and Medicinal Chemistry Division (NPMC), CSIR-Indian Institute of Integrative Medicine, Jammu, Jammu and Kashmir, India |
| Abstract: | The present work describes the synthesis, characterization, and wound healing properties of α/γ hybrid peptides: Boc-Phe-γ4-Phe-Val-OMe ( S1 ), Boc-DPhe-γ4-Phe-Val-OMe ( S2 ), Boc-Ala-γ4-Phe-Val-OMe ( S3 ), Boc-DAla-γ4-Phe-Val-OMe ( S4 ), Boc-Leu-γ4-Phe-Val-OMe ( S5 ), and Boc-DLeu-γ4-Phe-Val-OMe ( S6 ). Peptides S1–S6 were screened against human keratinocytes (HaCaT) and RAW 264.7 cells. Among all, S1 - and S2 -treated cells exhibited high cell viability; S1 and S2 induced keratinocyte migration and inhibited the production of the cytokines IL-6 and TNF-α. In vivo results demonstrated that the hybrid peptides S1 and S2 accelerate wound healing in Wistar rats with 83% and 88% at 50 μg/ml, and 74% and 76% at 25 μg/ml, respectively. |
| Keywords: | IL-6 keratinocyte migration TNF-α wound healing α/γ hybrid peptides γ4-phenylalanine |